Molecular detection of equine trypanosomiasis in the Riyadh Province of Saudi Arabia.
dc.contributor.author | Alanazi, AD | |
dc.contributor.author | Puschendorf, Robert | |
dc.contributor.author | Salim, B | |
dc.contributor.author | Alyousif, MS | |
dc.contributor.author | Alanazi, IO | |
dc.contributor.author | Al-Shehri, HR | |
dc.date.accessioned | 2018-10-16T10:21:39Z | |
dc.date.available | 2018-10-16T10:21:39Z | |
dc.date.issued | 2018-11 | |
dc.identifier.issn | 1040-6387 | |
dc.identifier.issn | 1943-4936 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/12551 | |
dc.description.abstract |
We conducted a cross-sectional study to detect trypanosome infections of horses and donkeys in the Riyadh Province of Saudi Arabia. DNA was extracted from blood samples collected from 368 horses and 142 donkeys, and subjected to universal first ribosomal internal transcribed spacer region (ITS1)-PCR followed by Trypanosoma evansi species-specific RoTat1.2-PCR. The universal ITS1-PCR revealed T. evansi infection in horses ( n = 12; 3.3%) and donkeys ( n = 4; 2.8%). There was no significant effect of sex or age on the prevalence of trypanosomiasis in horses or donkeys. Application of the RoTat1.2-PCR revealed that the RoTat1.2 VSG gene was absent from the positive ITS1-PCR samples of 3 horses and 1 donkey. This discrepancy could be explained by the circulation of T. evansi type B in Saudi Arabia; however, this suspicion requires confirmation. | |
dc.format.extent | 942-945 | |
dc.format.medium | Print-Electronic | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | SAGE Publications | |
dc.subject | Equine | |
dc.subject | ITS1 | |
dc.subject | PCR | |
dc.subject | RoTat1.2 | |
dc.subject | Saudi Arabia | |
dc.subject | trypanosomiasis | |
dc.title | Molecular detection of equine trypanosomiasis in the Riyadh Province of Saudi Arabia. | |
dc.type | journal-article | |
dc.type | Journal Article | |
plymouth.author-url | https://www.ncbi.nlm.nih.gov/pubmed/30204053 | |
plymouth.issue | 6 | |
plymouth.volume | 30 | |
plymouth.publication-status | Published | |
plymouth.journal | Journal of Veterinary Diagnostic Investigation | |
dc.identifier.doi | 10.1177/1040638718798688 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.publisher.place | United States | |
dcterms.dateAccepted | 2018-09-11 | |
dc.rights.embargodate | 2019-2-12 | |
dc.identifier.eissn | 1943-4936 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.1177/1040638718798688 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2018-11 | |
rioxxterms.type | Journal Article/Review |