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dc.contributor.authorLangan, Laura
dc.contributor.authorOwen, SF
dc.contributor.authorTrznadel, M
dc.contributor.authorDodd, NJF
dc.contributor.authorJackson, SK
dc.contributor.authorPurcell, WM
dc.contributor.authorJha, Awadhesh
dc.date.accessioned2018-09-13T11:25:03Z
dc.date.issued2018-08-22
dc.identifier.issn1663-9812
dc.identifier.issn1663-9812
dc.identifier.otherARTN 947
dc.identifier.urihttp://hdl.handle.net/10026.1/12349
dc.descriptionNo embargo required.
dc.description.abstract

Compared to two-dimensional (2D) cell culture, cellular aggregates or spheroids (3D) offer a more appropriate alternative in vitro system where individual cell-cell communication and micro-environment more closely represent the in vivo organ; yet we understand little of the physiological conditions at this scale. The relationship between spheroid size and oxygen microenvironment, an important factor influencing the metabolic capacity of cells, was first established using the fish intestine derived RTgutGC cell line. Subsequently, pharmaceutical metabolism (Propranolol), as determined by high performance liquid chromatography, in this intestinal model was examined as a function of spheroid size. Co-efficient of variation between spheroid size was below 12% using the gyratory platform method, with the least variation observed in the highest cell seeding density. The viable, high oxygen micro-environment of the outer rim of the spheroid, as determined by electron paramagnetic resonance (EPR) oximetry, decreased over time, and the hypoxic zone increased as a function of spheroid size. Despite a trend of higher metabolism in smaller spheroids, the formation of micro-environments (quiescent, hypoxic or anoxic) did not significantly affect metabolism or function of an environmentally relevant pharmaceutical in this spheroid model.

dc.format.extent947-
dc.format.mediumElectronic-eCollection
dc.languageeng
dc.language.isoen
dc.publisherFrontiers Media
dc.subjectspheroids
dc.subjectEPR-oximetry
dc.subjectmicro-environment
dc.subjectmetabolism
dc.subjectintestine
dc.subjectbeta-blocker
dc.subjectpropranolol
dc.titleSpheroid Size Does not Impact Metabolism of the beta-blocker Propranolol in 3D Intestinal Fish Model
dc.typejournal-article
dc.typeJournal Article
plymouth.author-urlhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000442393700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issueAUG
plymouth.volume9
plymouth.publication-statusPublished online
plymouth.journalFrontiers in Pharmacology
dc.identifier.doi10.3389/fphar.2018.00947
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Admin Group - REF
plymouth.organisational-group/Plymouth/Admin Group - REF/REF Admin Group - FoSE
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Research Groups/Marine Institute
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
plymouth.organisational-group/Plymouth/Users by role/Researchers in ResearchFish submission
dc.publisher.placeSwitzerland
dcterms.dateAccepted2018-08-02
dc.rights.embargodate2019-11-27
dc.identifier.eissn1663-9812
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.3389/fphar.2018.00947
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-08-22
rioxxterms.typeJournal Article/Review
plymouth.funderMoving up a dimension: 3D in vitro models as effective alternatives to live fish studies::BBSRC


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