Spheroid Size Does not Impact Metabolism of the beta-blocker Propranolol in 3D Intestinal Fish Model
dc.contributor.author | Langan, Laura | |
dc.contributor.author | Owen, SF | |
dc.contributor.author | Trznadel, M | |
dc.contributor.author | Dodd, NJF | |
dc.contributor.author | Jackson, SK | |
dc.contributor.author | Purcell, WM | |
dc.contributor.author | Jha, Awadhesh | |
dc.date.accessioned | 2018-09-13T11:25:03Z | |
dc.date.issued | 2018-08-22 | |
dc.identifier.issn | 1663-9812 | |
dc.identifier.issn | 1663-9812 | |
dc.identifier.other | ARTN 947 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/12349 | |
dc.description | No embargo required. | |
dc.description.abstract |
Compared to two-dimensional (2D) cell culture, cellular aggregates or spheroids (3D) offer a more appropriate alternative in vitro system where individual cell-cell communication and micro-environment more closely represent the in vivo organ; yet we understand little of the physiological conditions at this scale. The relationship between spheroid size and oxygen microenvironment, an important factor influencing the metabolic capacity of cells, was first established using the fish intestine derived RTgutGC cell line. Subsequently, pharmaceutical metabolism (Propranolol), as determined by high performance liquid chromatography, in this intestinal model was examined as a function of spheroid size. Co-efficient of variation between spheroid size was below 12% using the gyratory platform method, with the least variation observed in the highest cell seeding density. The viable, high oxygen micro-environment of the outer rim of the spheroid, as determined by electron paramagnetic resonance (EPR) oximetry, decreased over time, and the hypoxic zone increased as a function of spheroid size. Despite a trend of higher metabolism in smaller spheroids, the formation of micro-environments (quiescent, hypoxic or anoxic) did not significantly affect metabolism or function of an environmentally relevant pharmaceutical in this spheroid model. | |
dc.format.extent | 947- | |
dc.format.medium | Electronic-eCollection | |
dc.language | eng | |
dc.language.iso | en | |
dc.publisher | Frontiers Media | |
dc.subject | spheroids | |
dc.subject | EPR-oximetry | |
dc.subject | micro-environment | |
dc.subject | metabolism | |
dc.subject | intestine | |
dc.subject | beta-blocker | |
dc.subject | propranolol | |
dc.title | Spheroid Size Does not Impact Metabolism of the beta-blocker Propranolol in 3D Intestinal Fish Model | |
dc.type | journal-article | |
dc.type | Journal Article | |
plymouth.author-url | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000442393700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | AUG | |
plymouth.volume | 9 | |
plymouth.publication-status | Published online | |
plymouth.journal | Frontiers in Pharmacology | |
dc.identifier.doi | 10.3389/fphar.2018.00947 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Admin Group - REF | |
plymouth.organisational-group | /Plymouth/Admin Group - REF/REF Admin Group - FoSE | |
plymouth.organisational-group | /Plymouth/Faculty of Health | |
plymouth.organisational-group | /Plymouth/Faculty of Health/School of Biomedical Sciences | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science | |
plymouth.organisational-group | /Plymouth/Research Groups | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
plymouth.organisational-group | /Plymouth/Research Groups/Marine Institute | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
plymouth.organisational-group | /Plymouth/Users by role/Researchers in ResearchFish submission | |
dc.publisher.place | Switzerland | |
dcterms.dateAccepted | 2018-08-02 | |
dc.rights.embargodate | 2019-11-27 | |
dc.identifier.eissn | 1663-9812 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.versionofrecord | 10.3389/fphar.2018.00947 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.licenseref.startdate | 2018-08-22 | |
rioxxterms.type | Journal Article/Review | |
plymouth.funder | Moving up a dimension: 3D in vitro models as effective alternatives to live fish studies::BBSRC |