Measurement of Proton Leak in Isolated Mitochondria.
| dc.contributor.author | Affourtit, Charles | |
| dc.contributor.author | Wong, H-S | |
| dc.contributor.author | Brand, MD | |
| dc.date.accessioned | 2018-09-10T19:53:39Z | |
| dc.date.available | 2018-09-10T19:53:39Z | |
| dc.date.issued | 2018-05-23 | |
| dc.identifier.issn | 1064-3745 | |
| dc.identifier.issn | 1940-6029 | |
| dc.identifier.uri | http://hdl.handle.net/10026.1/12335 | |
| dc.description.abstract |
Oxidative phosphorylation is an important energy-conserving mechanism coupling mitochondrial electron transfer to ATP synthesis. Coupling between respiration and phosphorylation is not fully efficient due to proton leaks. In this chapter, we present a method to measure proton leak activity in isolated mitochondria. The relative strength of a modular kinetic approach to probe oxidative phosphorylation is emphasized. | |
| dc.format.extent | 157-170 | |
| dc.format.medium | ||
| dc.language | eng | |
| dc.language.iso | en | |
| dc.publisher | Humana Press (Springer Imprint) | |
| dc.subject | Membrane potential | |
| dc.subject | Mitochondria | |
| dc.subject | Modular kinetic analysis | |
| dc.subject | Oxidative phosphorylation | |
| dc.subject | Oxygen consumption | |
| dc.title | Measurement of Proton Leak in Isolated Mitochondria. | |
| dc.type | journal-article | |
| dc.type | Journal Article | |
| plymouth.author-url | https://www.ncbi.nlm.nih.gov/pubmed/29850999 | |
| plymouth.volume | 1782 | |
| plymouth.publication-status | Published | |
| plymouth.journal | Methods in Molecular Biology | |
| dc.identifier.doi | 10.1007/978-1-4939-7831-1_9 | |
| plymouth.organisational-group | /Plymouth | |
| plymouth.organisational-group | /Plymouth/Faculty of Health | |
| plymouth.organisational-group | /Plymouth/Faculty of Health/School of Biomedical Sciences | |
| plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
| plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
| plymouth.organisational-group | /Plymouth/Research Groups | |
| plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED) | |
| plymouth.organisational-group | /Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR | |
| plymouth.organisational-group | /Plymouth/Users by role | |
| plymouth.organisational-group | /Plymouth/Users by role/Academics | |
| dc.publisher.place | United States | |
| dcterms.dateAccepted | 2017-06-30 | |
| dc.rights.embargodate | 2019-5-23 | |
| dc.identifier.eissn | 1940-6029 | |
| dc.rights.embargoperiod | Not known | |
| rioxxterms.versionofrecord | 10.1007/978-1-4939-7831-1_9 | |
| rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
| rioxxterms.licenseref.startdate | 2018-05-23 | |
| rioxxterms.type | Journal Article/Review |
