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dc.contributor.authorGiner-Lamia, J
dc.contributor.authorHernández-Prieto, M
dc.contributor.authorE. Futschik, M
dc.date.accessioned2018-09-10T07:06:44Z
dc.date.issued2018-06-20
dc.identifier.issn2331-8325
dc.identifier.issn2331-8325
dc.identifier.otherARTN e2895
dc.identifier.urihttp://hdl.handle.net/10026.1/12254
dc.description.abstract

Nitrogen is an essential nutrient for all living organisms. In cyanobacteria, a group of oxygenic photosynthetic bacteria, nitrogen homeostasis is maintained by an intricate regulatory network around the transcription factor NtcA. Although mechanisms controlling NtcA activity appear to be well understood, the sets of genes under its control (i.e., its regulon) remain poorly defined. In this protocol, we describe the procedure for chromatin immunoprecipitation using NtcA antibodies, followed by DNA sequencing analysis (ChIP-seq) during early acclimation to nitrogen starvation in the cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis). This protocol can be extended to analyze any DNA-binding protein in cyanobacteria for which suitable antibodies exist.

dc.format.extente2895-
dc.format.mediumElectronic-eCollection
dc.languageen
dc.language.isoen
dc.publisherBio-Protocol
dc.subjectChIP-seq
dc.subjectCyanobacteria
dc.subjectSynechocystis
dc.subjectNitrogen
dc.subjectNtcA
dc.titleChIP-seq Experiment and Data Analysis in the Cyanobacterium Synechocystis sp. PCC 6803
dc.typejournal-article
dc.typeArticle
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/34286004
plymouth.issue12
plymouth.volume8
plymouth.publisher-urlhttp://dx.doi.org/10.21769/bioprotoc.2895
plymouth.publication-statusPublished
plymouth.journalBio-protocol
dc.identifier.doi10.21769/BioProtoc.2895
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/Users by role
dc.publisher.placeUnited States
dcterms.dateAccepted2018-05-17
dc.rights.embargodate2018-9-19
dc.identifier.eissn2331-8325
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.21769/BioProtoc.2895
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-06-20
rioxxterms.typeJournal Article/Review


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