Show simple item record

dc.contributor.authorSassmann, Sen
dc.contributor.authorRodrigues, Cen
dc.contributor.authorMilne, SWen
dc.contributor.authorNenninger, Aen
dc.contributor.authorAllwood, Een
dc.contributor.authorLittlejohn, GRen
dc.contributor.authorTalbot, NJen
dc.contributor.authorSoeller, Cen
dc.contributor.authorDavies, Ben
dc.contributor.authorHussey, PJen
dc.contributor.authorDeeks, MJen
dc.date.accessioned2018-07-19T12:17:28Z
dc.date.issued2018-07-09en
dc.identifier.urihttp://hdl.handle.net/10026.1/11856
dc.description.abstract

Cell wall appositions (CWAs) are produced reactively by the plant immune system to arrest microbial invasion through the local inversion of plant cell growth. This process requires the controlled invagination of the plasma membrane (PM) in coordination with the export of barrier material to the volume between the plant PM and cell wall. Plant actin dynamics are essential to this response, but it remains unclear how exocytosis and the cytoskeleton are linked in space and time to form functional CWAs. Here, we show that actin-dependent trafficking to immune response sites of Arabidopsis thaliana delivers membrane-integrated FORMIN4, which in turn contributes to local cytoskeletal dynamics. Total internal reflection fluorescence (TIRF) microscopy combined with controlled induction of FORMIN4-GFP expression reveals a dynamic population of vesicular bodies that accumulate to form clusters at the PM through an actin-dependent process. Deactivation of FORMIN4 and its close homologs partially compromises subsequent defense and alters filamentous actin (F-actin) distribution at mature CWAs. The localization of FORMIN4 is stable and segregated from the dynamic traffic of the endosomal network. Moreover, the tessellation of FORMIN4 at the PM with meso-domains of PEN3 reveals a fine spatial segregation of destinations for actin-dependent immunity cargo. Together, our data suggest a model where FORMIN4 is a spatial feedback element in a multi-layered, temporally defined sequence of cytoskeletal response. This positional feedback makes a significant contribution to the distribution of actin filaments at the dynamic CWA boundary and to the outcomes of pre-invasion defense.

en
dc.format.extent2136 - 2144.e7en
dc.language.isoenen
dc.subjectactinen
dc.subjectcalloseen
dc.subjectcytoskeletonen
dc.subjectdefenseen
dc.subjectforminen
dc.subjectimmunityen
dc.subjectpen3en
dc.subjectplanten
dc.subjectpowdery mildewen
dc.subjectvesicleen
dc.titleAn Immune-Responsive Cytoskeletal-Plasma Membrane Feedback Loop in Plants.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/29937351en
plymouth.issue13en
plymouth.volume28en
plymouth.journalCurr Biolen
dc.identifier.doi10.1016/j.cub.2018.05.014en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dcterms.dateAccepted2018-05-04en
dc.rights.embargodate2018-07-21en
dc.identifier.eissn1879-0445en
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1016/j.cub.2018.05.014en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-07-09en
rioxxterms.typeJournal Article/Reviewen


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record


All items in PEARL are protected by copyright law.
Author manuscripts deposited to comply with open access mandates are made available in accordance with publisher policies. Please cite only the published version using the details provided on the item record or document. In the absence of an open licence (e.g. Creative Commons), permissions for further reuse of content should be sought from the publisher or author.
Theme by 
@mire NV