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dc.contributor.authorChristel, Sen
dc.contributor.authorHerold, Men
dc.contributor.authorBellenberg, Sen
dc.contributor.authorEl Hajjami, Men
dc.contributor.authorBuetti-Dinh, Aen
dc.contributor.authorPivkin, IVen
dc.contributor.authorSand, Wen
dc.contributor.authorWilmes, Pen
dc.contributor.authorPoetsch, Aen
dc.contributor.authorDopson, Men
dc.date.accessioned2018-07-11T15:49:25Z
dc.date.available2018-07-11T15:49:25Z
dc.date.issued2018-02-01en
dc.identifier.urihttp://hdl.handle.net/10026.1/11829
dc.description.abstract

Leptospirillum ferriphilum plays a major role in acidic, metal-rich environments, where it represents one of the most prevalent iron oxidizers. These milieus include acid rock and mine drainage as well as biomining operations. Despite its perceived importance, no complete genome sequence of the type strain of this model species is available, limiting the possibilities to investigate the strategies and adaptations that Leptospirillum ferriphilum DSM 14647T (here referred to as Leptospirillum ferriphilum T) applies to survive and compete in its niche. This study presents a complete, circular genome of Leptospirillum ferriphilum T obtained by PacBio single-molecule real-time (SMRT) long-read sequencing for use as a high-quality reference. Analysis of the functionally annotated genome, mRNA transcripts, and protein concentrations revealed a previously undiscovered nitrogenase cluster for atmospheric nitrogen fixation and elucidated metabolic systems taking part in energy conservation, carbon fixation, pH homeostasis, heavy metal tolerance, the oxidative stress response, chemotaxis and motility, quorum sensing, and biofilm formation. Additionally, mRNA transcript counts and protein concentrations were compared between cells grown in continuous culture using ferrous iron as the substrate and those grown in bioleaching cultures containing chalcopyrite (CuFeS2). Adaptations of Leptospirillum ferriphilum T to growth on chalcopyrite included the possibly enhanced production of reducing power, reduced carbon dioxide fixation, as well as elevated levels of RNA transcripts and proteins involved in heavy metal resistance, with special emphasis on copper efflux systems. Finally, the expression and translation of genes responsible for chemotaxis and motility were enhanced.IMPORTANCE Leptospirillum ferriphilum is one of the most important iron oxidizers in the context of acidic and metal-rich environments during moderately thermophilic biomining. A high-quality circular genome of Leptospirillum ferriphilum T coupled with functional omics data provides new insights into its metabolic properties, such as the novel identification of genes for atmospheric nitrogen fixation, and represents an essential step for further accurate proteomic and transcriptomic investigation of this acidophile model species in the future. Additionally, light is shed on adaptation strategies of Leptospirillum ferriphilum T for growth on the copper mineral chalcopyrite. These data can be applied to deepen our understanding and optimization of bioleaching and biooxidation, techniques that present sustainable and environmentally friendly alternatives to many traditional methods for metal extraction.

en
dc.languageengen
dc.language.isoengen
dc.subjectLeptospirillumen
dc.subjectacidophileen
dc.subjectbiominingen
dc.subjectgenomeen
dc.subjectmetabolismen
dc.subjectomicsen
dc.subjectproteomeen
dc.subjecttranscriptomeen
dc.subjectBacteriaen
dc.subjectCopperen
dc.subjectGenome, Bacterialen
dc.subjectHydrogen-Ion Concentrationen
dc.subjectIronen
dc.subjectOxidation-Reductionen
dc.subjectPhylogenyen
dc.subjectProteomeen
dc.subjectProteomicsen
dc.subjectRNA, Bacterialen
dc.subjectTranscriptomeen
dc.titleMulti-omics Reveals the Lifestyle of the Acidophilic, Mineral-Oxidizing Model Species Leptospirillum ferriphilumT.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/29150517en
plymouth.issue3en
plymouth.volume84en
plymouth.publication-statusPublished onlineen
plymouth.journalAppl Environ Microbiolen
dc.identifier.doi10.1128/AEM.02091-17en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
dc.publisher.placeUnited Statesen
dcterms.dateAccepted2017-11-09en
dc.identifier.eissn1098-5336en
dc.rights.embargoperiodNo embargoen
rioxxterms.versionofrecord10.1128/AEM.02091-17en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-02-01en
rioxxterms.typeJournal Article/Reviewen


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