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dc.contributor.authorDesriac, Fen
dc.contributor.authorClamens, Ten
dc.contributor.authorRosay, Ten
dc.contributor.authorRodrigues, Sen
dc.contributor.authorTahrioui, Aen
dc.contributor.authorEnault, Jen
dc.contributor.authorRoquigny, Len
dc.contributor.authorRacine, P-Jen
dc.contributor.authorTaupin, Len
dc.contributor.authorBazire, Aen
dc.contributor.authorDufour, Aen
dc.contributor.authorLeprince, Jen
dc.contributor.authorBouffartigues, Een
dc.contributor.authorChevalier, Sen
dc.contributor.authorFeuilloley, MGJen
dc.contributor.authorLesouhaitier, Oen
dc.date.accessioned2018-07-06T08:38:54Z
dc.date.issued2018-04-24en
dc.identifier.issn2076-0817en
dc.identifier.urihttp://hdl.handle.net/10026.1/11806
dc.description.abstract

We have previously shown that the C-type Natriuretic Peptide (CNP), a peptide produced by lungs, is able to impact Pseudomonasaeruginosa physiology. In the present work, the effect of CNP at different concentrations on P. aeruginosa biofilm formation was studied and the mechanisms of action of this human hormone on P. aeruginosa were deciphered. CNP was shown to inhibit dynamic biofilm formation in a dose-dependent manner without affecting the bacterial growth at any tested concentrations. The most effective concentrations were 1 and 0.1 µM. At 0.1 µM, the biofilm formation inhibition was fully dependent on the CNP sensor protein AmiC, whereas it was only partially AmiC-dependent at 1 µM, revealing the existence of a second AmiC-independent mode of action of CNP on P. aeruginosa. At 1 µM, CNP reduced both P. aeruginosa adhesion on glass and di-rhamnolipid production and also increased the bacterial membrane fluidity. The various effects of CNP at 1 µM and 0.1 µM on P. aeruginosa shown here should have major consequences to design drugs for biofilm treatment or prevention.

en
dc.language.isoenen
dc.subjectbacterial adaptationen
dc.subjectbiofilmen
dc.subjectcystic fibrosisen
dc.subjecthormonesen
dc.subjectlungen
dc.subjectpeptidesen
dc.titleDifferent Dose-Dependent Modes of Action of C-Type Natriuretic Peptide on Pseudomonas aeruginosa Biofilm Formation.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/29695043en
plymouth.issue2en
plymouth.volume7en
plymouth.journalPathogensen
dc.identifier.doi10.3390/pathogens7020047en
plymouth.organisational-group/Plymouth
dcterms.dateAccepted2018-04-20en
dc.rights.embargodate2018-07-10en
dc.rights.embargoperiodNo embargoen
rioxxterms.versionofrecord10.3390/pathogens7020047en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-04-24en
rioxxterms.typeJournal Article/Reviewen


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