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dc.contributor.authorKos, K
dc.contributor.authorWong, S
dc.contributor.authorTan, B
dc.contributor.authorGummesson, A
dc.contributor.authorJernas, M
dc.contributor.authorFranck, N
dc.contributor.authorKerrigan, D
dc.contributor.authorNystrom, FH
dc.contributor.authorCarlsson, LMS
dc.contributor.authorRandeva, HS
dc.contributor.authorPinkney, Jonathan
dc.contributor.authorWilding, JPH
dc.date.accessioned2018-04-25T13:52:46Z
dc.date.available2018-04-25T13:52:46Z
dc.date.issued2009-08-01
dc.identifier.issn0012-1797
dc.identifier.issn1939-327X
dc.identifier.urihttp://hdl.handle.net/10026.1/11345
dc.description.abstract

<jats:sec> <jats:title>OBJECTIVE</jats:title> <jats:p>Matricellular Secreted Protein, Acidic and Rich in Cysteine (SPARC), originally discovered in bone as osteonectin, is a mediator of collagen deposition and promotes fibrosis. Adipose tissue collagen has recently been found to be linked with metabolic dysregulation. Therefore, we tested the hypothesis that SPARC in human adipose tissue is influenced by glucose metabolism and adipokines.</jats:p> </jats:sec> <jats:sec> <jats:title>RESEARCH DESIGN AND METHODS</jats:title> <jats:p>Serum and adipose tissue biopsies were obtained from morbidly obese nondiabetic subjects undergoing bariatric surgery and lean control subjects for analysis of metabolic markers, SPARC, and various cytokines (RT-PCR). Additionally, 24 obese subjects underwent a very-low-calorie diet of 1,883 kJ (450 kcal)/day for 16 weeks and serial subcutaneous-abdominal-adipose tissue (SCAT) biopsies (weight loss: 28 ± 3.7 kg). Another six lean subjects underwent fast-food–based hyperalimentation for 4 weeks (weight gain: 7.2 ± 1.6 kg). Finally, visceral adipose tissue explants were cultured with recombinant leptin, insulin, and glucose, and SPARC mRNA and protein expression determined by Western blot analyses.</jats:p> </jats:sec> <jats:sec> <jats:title>RESULTS</jats:title> <jats:p>SPARC expression in human adipose tissue correlated with fat mass and was higher in SCAT. Weight loss induced by very-low-calorie diet lowered SPARC expression by 33% and increased by 30% in adipose tissue of subjects gaining weight after a fast-food diet. SPARC expression was correlated with leptin independent of fat mass and correlated with homeostasis model assessment–insulin resistance. In vitro experiments showed that leptin and insulin potently increased SPARC production dose dependently in visceral adipose tissue explants, while glucose decreased SPARC protein.</jats:p> </jats:sec> <jats:sec> <jats:title>CONCLUSIONS</jats:title> <jats:p>Our data suggest that SPARC expression is predominant in subcutaneous fat and its expression and secretion in adipose tissue are influenced by fat mass, leptin, insulin, and glucose. The profibrotic effects of SPARC may contribute to metabolic dysregulation in obesity.</jats:p> </jats:sec>

dc.format.extent1780-1788
dc.format.mediumPrint-Electronic
dc.language.isoen
dc.publisherAmerican Diabetes Association
dc.subjectAdipose Tissue
dc.subjectAdult
dc.subjectBariatric Surgery
dc.subjectBlood Glucose
dc.subjectBlood Pressure
dc.subjectBody Mass Index
dc.subjectBody Weight
dc.subjectDiet, Reducing
dc.subjectFemale
dc.subjectGene Expression Regulation
dc.subjectHumans
dc.subjectInsulin
dc.subjectLeptin
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectNeovascularization, Physiologic
dc.subjectObesity
dc.subjectObesity, Morbid
dc.subjectOsteonectin
dc.titleRegulation of the Fibrosis and Angiogenesis Promoter SPARC/Osteonectin in Human Adipose Tissue by Weight Change, Leptin, Insulin, and Glucose
dc.typeconference
dc.typeJournal Article
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000268610300011&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue8
plymouth.volume58
plymouth.publication-statusPublished
plymouth.journalDiabetes
dc.identifier.doi10.2337/db09-0211
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/Peninsula Medical School
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
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plymouth.organisational-group/Plymouth/Research Groups/FoH - Community and Primary Care
plymouth.organisational-group/Plymouth/Research Groups/Institute of Health and Community
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CCT&PS
plymouth.organisational-group/Plymouth/Research Groups/Plymouth Institute of Health and Care Research (PIHR)
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dc.publisher.placeUnited States
dc.identifier.eissn1939-327X
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.2337/db09-0211
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeConference Paper/Proceeding/Abstract


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