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dc.contributor.authorAlRabiah, H
dc.contributor.authorCorrea, E
dc.contributor.authorUpton, M
dc.contributor.authorGoodacre, R
dc.date.accessioned2018-03-21T09:47:17Z
dc.date.available2018-03-21T09:47:17Z
dc.date.issued2013
dc.identifier.issn0003-2654
dc.identifier.issn1364-5528
dc.identifier.urihttp://hdl.handle.net/10026.1/11127
dc.description.abstract

Fourier transform infrared (FT-IR) spectroscopy is an established rapid whole-organism fingerprinting method that generates metabolic fingerprints from bacteria that reflect the phenotype of the microorganism under investigation. However, whilst FT-IR spectroscopy is fast (typically 10 s to 1 min per sample), the approaches for microbial sample preparation can be time consuming as plate culture or shake flasks are used for growth of the organism. We report a new approach that allows micro-cultivation of bacteria from low volumes (typically 200 μL) to be coupled with FT-IR spectroscopy. This approach is fast and easy to perform and gives equivalent data to the lengthier and more expensive shake flask cultivations (sample volume = 20 mL). With this micro-culture approach we also demonstrate high reproducibility of the metabolic fingerprints. The approach allowed separation of different isolates of Escherichia coli involved in urinary tract infection, including members of the globally disseminated ST131 clone, with respect to both genotype and resistance or otherwise to the antibiotic Ciprofloxacin.

dc.format.extent1363-1363
dc.format.mediumPrint
dc.languageen
dc.language.isoeng
dc.publisherRoyal Society of Chemistry (RSC)
dc.subjectBacterial Typing Techniques
dc.subjectEscherichia coli
dc.subjectEscherichia coli Infections
dc.subjectHigh-Throughput Screening Assays
dc.subjectHumans
dc.subjectReproducibility of Results
dc.subjectSample Size
dc.subjectSpectroscopy, Fourier Transform Infrared
dc.subjectTime Factors
dc.titleHigh-throughput phenotyping of uropathogenic E. coli isolates with Fourier transform infrared spectroscopy
dc.typejournal-article
dc.typeArticle
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/23325321
plymouth.issue5
plymouth.volume138
plymouth.publisher-urlhttp://dx.doi.org/10.1039/c3an36517d
plymouth.publication-statusPublished
plymouth.journalThe Analyst
dc.identifier.doi10.1039/c3an36517d
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Research Groups/Plymouth Institute of Health and Care Research (PIHR)
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
plymouth.organisational-group/Plymouth/Users by role/Researchers in ResearchFish submission
dc.publisher.placeEngland
dc.identifier.eissn1364-5528
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1039/c3an36517d
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review


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