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dc.contributor.authorDutia, BM
dc.contributor.authorReid, SJ
dc.contributor.authorDrummond, DD
dc.contributor.authorLigertwood, Y
dc.contributor.authorBennet, I
dc.contributor.authorRietberg, W
dc.contributor.authorSilvia, O
dc.contributor.authorJarvis, Michael A
dc.contributor.authorNash, AA
dc.date.accessioned2018-03-06T19:43:43Z
dc.date.available2018-03-06T19:43:43Z
dc.date.issued2009-08-04
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.otherARTN e6492
dc.identifier.urihttp://hdl.handle.net/10026.1/10994
dc.description.abstract

BACKGROUND: Detection, isolation, and identification of individual virus infected cells during long term infection are critical to advance our understanding of mechanisms of pathogenesis for latent/persistent viruses. However, current approaches to study these viruses in vivo have been hampered by low sensitivity and effects of cell-type on expression of viral encoded reporter genes. We have designed a novel Cre recombinase (Cre)-based murine system to overcome these problems, and thereby enable tracking and isolation of individual in vivo infected cells. METHODOLOGY/PRINCIPAL FINDINGS: Murine gammaherpesvirus 68 (MHV-68) was used as a prototypic persistent model virus. A Cre expressing recombinant virus was constructed and characterised. The virus is attenuated both in lytic virus replication, producing ten-fold lower lung virus titres than wild type virus, and in the establishment of latency. However, despite this limitation, when the sEGFP7 mouse line containing a Cre-activated enhanced green fluorescent protein (EGFP) was infected with the Cre expressing virus, sites of latent and persistent virus infection could be identified within B cells and macrophages of the lymphoid system on the basis of EGFP expression. Importantly, the use of the sEGFP7 mouse line which expresses high levels of EGFP allowed individual virus positive cells to be purified by FACSorting. Virus gene expression could be detected in these cells. Low numbers of EGFP positive cells could also be detected in the bone marrow. CONCLUSIONS/SIGNIFICANCE: The use of this novel Cre-based virus/mouse system allowed identification of individual latently infected cells in vivo and may be useful for the study and long-term monitoring of other latent/persistent virus infections.

dc.format.extente6492-e6492
dc.format.mediumElectronic
dc.languageen
dc.language.isoeng
dc.publisherPublic Library of Science (PLoS)
dc.subjectAnimals
dc.subjectB-Lymphocytes
dc.subjectBase Sequence
dc.subjectBlotting, Southern
dc.subjectCell Line
dc.subjectCricetinae
dc.subjectFlow Cytometry
dc.subjectGammaherpesvirinae
dc.subjectGreen Fluorescent Proteins
dc.subjectHerpesviridae Infections
dc.subjectImmunohistochemistry
dc.subjectIntegrases
dc.subjectMacrophages
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectMice, Inbred C57BL
dc.subjectMolecular Sequence Data
dc.subjectPolymerase Chain Reaction
dc.subjectRecombination, Genetic
dc.titleA Novel Cre Recombinase Imaging System for Tracking Lymphotropic Virus Infection In Vivo
dc.typejournal-article
dc.typeJournal Article
dc.typeResearch Support, N.I.H., Extramural
dc.typeResearch Support, Non-U.S. Gov't
plymouth.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000268637800006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008
plymouth.issue8
plymouth.volume4
plymouth.publication-statusPublished online
plymouth.journalPLoS ONE
dc.identifier.doi10.1371/journal.pone.0006492
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Health
plymouth.organisational-group/Plymouth/Faculty of Health/School of Biomedical Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/Research Groups
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)
plymouth.organisational-group/Plymouth/Research Groups/Institute of Translational and Stratified Medicine (ITSMED)/CBR
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeUnited States
dcterms.dateAccepted2009-07-06
dc.identifier.eissn1932-6203
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1371/journal.pone.0006492
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2009-08-04
rioxxterms.typeJournal Article/Review


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