Development of a Label-Free Immunosensor for Clusterin Detection as an Alzheimer’s Biomarker
dc.contributor.author | Islam, K | |
dc.contributor.author | Damiati, S | |
dc.contributor.author | Sethi, J | |
dc.contributor.author | Suhail, A | |
dc.contributor.author | Pan, Genhua | |
dc.date.accessioned | 2018-02-20T15:43:05Z | |
dc.date.available | 2018-02-20T15:43:05Z | |
dc.date.issued | 2018-01 | |
dc.identifier.issn | 1424-8220 | |
dc.identifier.issn | 1424-8220 | |
dc.identifier.other | 308 | |
dc.identifier.uri | http://hdl.handle.net/10026.1/10827 | |
dc.description.abstract |
Clusterin (CLU) has been associated with the clinical progression of Alzheimer's disease (AD) and described as a potential AD biomarker in blood plasma. Due to the enormous attention given to cerebrospinal fluid (CSF) biomarkers for the past couple of decades, recently found blood-based AD biomarkers like CLU have not yet been reported for biosensors. Herein, we report the electrochemical detection of CLU for the first time using a screen-printed carbon electrode (SPCE) modified with 1-pyrenebutyric acid N-hydroxysuccinimide ester (Pyr-NHS) and decorated with specific anti-CLU antibody fragments. This bifunctional linker molecule contains succinylimide ester to bind protein at one end while its pyrene moiety attaches to the carbon surface by means of π-π stacking. Cyclic voltammetric and square wave voltammetric studies showed the limit of detection down to 1 pg/mL and a linear concentration range of 1-100 pg/mL with good sensitivity. Detection of CLU in spiked human plasma was demonstrated with satisfactory recovery percentages to that of the calibration data. The proposed method facilitates the cost-effective and viable production of label-free point-of-care devices for the clinical diagnosis of AD. | |
dc.format.extent | 0-0 | |
dc.format.medium | Electronic | |
dc.language | en | |
dc.language.iso | en | |
dc.publisher | MDPI AG | |
dc.subject | clusterin | |
dc.subject | electrochemical biosensor | |
dc.subject | label-free immunosensor | |
dc.subject | cyclic voltammetry | |
dc.subject | square wave voltammetry | |
dc.subject | screen-printed electrodes | |
dc.subject | Alzheimer's disease | |
dc.title | Development of a Label-Free Immunosensor for Clusterin Detection as an Alzheimer’s Biomarker | |
dc.type | journal-article | |
dc.type | Journal Article | |
plymouth.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000423286300306&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=11bb513d99f797142bcfeffcc58ea008 | |
plymouth.issue | 1 | |
plymouth.volume | 18 | |
plymouth.publication-status | Published online | |
plymouth.journal | Sensors | |
dc.identifier.doi | 10.3390/s18010308 | |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering | |
plymouth.organisational-group | /Plymouth/Faculty of Science and Engineering/School of Engineering, Computing and Mathematics | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA12 Engineering | |
plymouth.organisational-group | /Plymouth/Users by role | |
plymouth.organisational-group | /Plymouth/Users by role/Academics | |
dc.publisher.place | Switzerland | |
dcterms.dateAccepted | 2018-01-18 | |
dc.identifier.eissn | 1424-8220 | |
dc.rights.embargoperiod | Not known | |
rioxxterms.funder | EPSRC | |
rioxxterms.identifier.project | Novel Point-of-Care Diagnostic Techniques for Dementia | |
rioxxterms.versionofrecord | 10.3390/s18010308 | |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | |
rioxxterms.type | Journal Article/Review | |
plymouth.funder | Novel Point-of-Care Diagnostic Techniques for Dementia::EPSRC |