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dc.contributor.authorSambles, C
dc.contributor.authorMoore, K
dc.contributor.authorLux, TM
dc.contributor.authorJones, K
dc.contributor.authorLittlejohn, GR
dc.contributor.authorGouveia, JD
dc.contributor.authorAves, SJ
dc.contributor.authorStudholme, DJ
dc.contributor.authorLee, R
dc.contributor.authorLove, J
dc.date.accessioned2018-02-09T16:15:25Z
dc.date.available2018-02-09T16:15:25Z
dc.date.issued2017-08
dc.identifier.issn2045-8827
dc.identifier.issn2045-8827
dc.identifier.otherARTN e482
dc.identifier.urihttp://hdl.handle.net/10026.1/10761
dc.description.abstract

<jats:title>Abstract</jats:title><jats:p>Microalgae are widely viewed as a promising and sustainable source of renewable chemicals and biofuels. <jats:italic>Botryococcus braunii</jats:italic> synthesizes and secretes significant amounts of long‐chain (C<jats:sub>30</jats:sub>‐C<jats:sub>40</jats:sub>) hydrocarbons that can be subsequently converted into gasoline, diesel, and aviation fuel. <jats:italic>B. braunii</jats:italic> cultures are not axenic and the effects of co‐cultured microorganisms on <jats:italic>B. braunii</jats:italic> growth and hydrocarbon yield are important, but sometimes contradictory. To understand the composition of the <jats:italic>B. braunii</jats:italic> microbial consortium, we used high throughput Illumina sequencing of metagenomic <jats:styled-content style="fixed-case">DNA</jats:styled-content> to profile the microbiota within a well established, stable B<jats:italic>. braunii</jats:italic> culture and characterized the demographic changes in the microcosm following modification to the culture conditions. <jats:styled-content style="fixed-case">DNA</jats:styled-content> sequences attributed to <jats:italic>B. braunii</jats:italic> were present in equal quantities in all treatments, whereas sequences assigned to the associated microbial community were dramatically altered. Bacterial species least affected by treatments, and more robustly associated with the algal cells, included members of Rhizobiales, comprising <jats:italic>Bradyrhizobium</jats:italic> and <jats:italic>Methylobacterium</jats:italic>, and representatives of <jats:italic>Dyadobacter</jats:italic>,<jats:italic> Achromobacter</jats:italic> and <jats:italic>Asticcacaulis</jats:italic>. The presence of bacterial species identified by metagenomics was confirmed by additional 16S r<jats:styled-content style="fixed-case">DNA</jats:styled-content> analysis of bacterial isolates. Our study demonstrates the advantages of high throughput sequencing and robust metagenomic analyses to define microcosms and further our understanding of microbial ecology.</jats:p>

dc.format.extente00482-
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoeng
dc.publisherWiley
dc.subjectbiofuel
dc.subjectBotryococcus braunii
dc.subjectconsortium
dc.subjectmetagenomics
dc.subjectmicrocosm
dc.titleMetagenomic analysis of the complex microbial consortium associated with cultures of the oil‐rich alga <i>Botryococcus braunii</i>
dc.typejournal-article
dc.typeArticle
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/28660691
plymouth.issue4
plymouth.volume6
plymouth.publisher-urlhttp://dx.doi.org/10.1002/mbo3.482
plymouth.publication-statusPublished
plymouth.journalMicrobiologyOpen
dc.identifier.doi10.1002/mbo3.482
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering/School of Biological and Marine Sciences
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
plymouth.organisational-group/Plymouth/Users by role
plymouth.organisational-group/Plymouth/Users by role/Academics
dc.publisher.placeEngland
dcterms.dateAccepted2017-03-14
dc.identifier.eissn2045-8827
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1002/mbo3.482
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2017-08
rioxxterms.typeJournal Article/Review


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