Molecular cloning of a gene encoding a 20S proteasome beta subunit from Plasmodium falciparum.
Date
2000-05Author
Li, GD
Li, JL
Mugthin, M
Ward, SA
Subject
Adenosine Triphosphatases Amino Acid Sequence Animals Cloning, Molecular Cysteine Endopeptidases Humans Mice Molecular Sequence Data Multienzyme Complexes Plasmodium falciparum Proteasome Endopeptidase Complex Protein Conformation Rats Sequence Alignment Xenopus laevis Zebrafish
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Show full item recordAbstract
A novel gene was cloned from Plasmodium falciparum. Database searches indicated this gene to be a member of the 20S proteasome beta-subunit family. Comparison of the gene's genomic DNA sequence with cDNA sequence revealed a 156-bp intron 85 bp downstream from the start codon. The nucleotide sequence of the gene contains one open reading frame encoding 265 amino acids with a predicted molecular mass of 30.9 kDa and a pI of 6.2. Northern blot analysis showed the transcript size to be approximately 1.6 kb indicating that some 800 bp of the transcript is non-coding.
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Place of Publication
England
Journal
Int J Parasitol
Volume
30
Issue
6
Pagination
729 - 733
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