Pfmrk, a MO15-related protein kinase from Plasmodium falciparum. Gene cloning, sequence, stage-specific expression and chromosome localization.
dc.contributor.author | Li, JL | en |
dc.contributor.author | Robson, KJ | en |
dc.contributor.author | Chen, JL | en |
dc.contributor.author | Targett, GA | en |
dc.contributor.author | Baker, DA | en |
dc.date.accessioned | 2017-11-27T18:53:36Z | |
dc.date.available | 2017-11-27T18:53:36Z | |
dc.date.issued | 1996-11-01 | en |
dc.identifier.issn | 0014-2956 | en |
dc.identifier.uri | http://hdl.handle.net/10026.1/10329 | |
dc.description.abstract |
Cyclin-dependent kinases (Cdks) play a central role in the regulation of the eukaryotic cell cycle. A novel gene encoding a Cdk-like protein, Pfmrk, has been isolated from the human malaria parasite Plasmodium falciparum. The gene has no introns and comprises an open reading frame encoding a protein of 324 amino acids with a predicted molecular mass of 38 kDa. Database searches revealed a striking similarity to the Cdk subfamily with the highest similarity to human MO15 (Cdk7). The overall sequence of Pfmrk shares 62% similarity and 46% identity with human MO15, in comparison to the 49-58% similarity and 34-43% identity with other human Cdks. Pfmrk contains two unique inserts: one consisting of 5 amino acids just before the cyclin-binding motif and the other composed of 13 amino acids within the T-loop equivalent region. Southern blots of genomic DNA digests and chromosomal separations showed that Pfmrk is a single-copy gene conserved between several parasite strains and is located on chromosome 10. A 2500-nucleotide transcript of this gene is expressed predominantly in the sexual blood stages (gametocytes), suggesting that Pfmrk may be involved in sexual stage development. | en |
dc.format.extent | 805 - 813 | en |
dc.language | eng | en |
dc.language.iso | eng | en |
dc.subject | Amino Acid Sequence | en |
dc.subject | Animals | en |
dc.subject | Base Sequence | en |
dc.subject | Chromosome Mapping | en |
dc.subject | Cloning, Molecular | en |
dc.subject | Cyclin-Dependent Kinases | en |
dc.subject | Gene Dosage | en |
dc.subject | Genes, Protozoan | en |
dc.subject | Molecular Sequence Data | en |
dc.subject | Plasmodium falciparum | en |
dc.subject | Protein Kinases | en |
dc.subject | Protein Serine-Threonine Kinases | en |
dc.subject | Protozoan Proteins | en |
dc.subject | RNA, Messenger | en |
dc.subject | RNA, Protozoan | en |
dc.subject | Sequence Analysis, DNA | en |
dc.subject | Sequence Homology, Amino Acid | en |
dc.title | Pfmrk, a MO15-related protein kinase from Plasmodium falciparum. Gene cloning, sequence, stage-specific expression and chromosome localization. | en |
dc.type | Journal Article | |
plymouth.author-url | https://www.ncbi.nlm.nih.gov/pubmed/8944769 | en |
plymouth.issue | 3 | en |
plymouth.volume | 241 | en |
plymouth.publication-status | Published | en |
plymouth.journal | Eur J Biochem | en |
dc.identifier.doi | 10.1111/j.1432-1033.1996.00805.x | en |
plymouth.organisational-group | /Plymouth | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine | |
plymouth.organisational-group | /Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine | |
dc.publisher.place | England | en |
dc.rights.embargoperiod | Not known | en |
rioxxterms.versionofrecord | 10.1111/j.1432-1033.1996.00805.x | en |
rioxxterms.licenseref.uri | http://www.rioxx.net/licenses/all-rights-reserved | en |
rioxxterms.type | Journal Article/Review | en |