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dc.contributor.authorLi, JLen
dc.contributor.authorRobson, KJen
dc.contributor.authorChen, JLen
dc.contributor.authorTargett, GAen
dc.contributor.authorBaker, DAen
dc.date.accessioned2017-11-27T18:53:36Z
dc.date.available2017-11-27T18:53:36Z
dc.date.issued1996-11-01en
dc.identifier.issn0014-2956en
dc.identifier.urihttp://hdl.handle.net/10026.1/10329
dc.description.abstract

Cyclin-dependent kinases (Cdks) play a central role in the regulation of the eukaryotic cell cycle. A novel gene encoding a Cdk-like protein, Pfmrk, has been isolated from the human malaria parasite Plasmodium falciparum. The gene has no introns and comprises an open reading frame encoding a protein of 324 amino acids with a predicted molecular mass of 38 kDa. Database searches revealed a striking similarity to the Cdk subfamily with the highest similarity to human MO15 (Cdk7). The overall sequence of Pfmrk shares 62% similarity and 46% identity with human MO15, in comparison to the 49-58% similarity and 34-43% identity with other human Cdks. Pfmrk contains two unique inserts: one consisting of 5 amino acids just before the cyclin-binding motif and the other composed of 13 amino acids within the T-loop equivalent region. Southern blots of genomic DNA digests and chromosomal separations showed that Pfmrk is a single-copy gene conserved between several parasite strains and is located on chromosome 10. A 2500-nucleotide transcript of this gene is expressed predominantly in the sexual blood stages (gametocytes), suggesting that Pfmrk may be involved in sexual stage development.

en
dc.format.extent805 - 813en
dc.languageengen
dc.language.isoengen
dc.subjectAmino Acid Sequenceen
dc.subjectAnimalsen
dc.subjectBase Sequenceen
dc.subjectChromosome Mappingen
dc.subjectCloning, Molecularen
dc.subjectCyclin-Dependent Kinasesen
dc.subjectGene Dosageen
dc.subjectGenes, Protozoanen
dc.subjectMolecular Sequence Dataen
dc.subjectPlasmodium falciparumen
dc.subjectProtein Kinasesen
dc.subjectProtein Serine-Threonine Kinasesen
dc.subjectProtozoan Proteinsen
dc.subjectRNA, Messengeren
dc.subjectRNA, Protozoanen
dc.subjectSequence Analysis, DNAen
dc.subjectSequence Homology, Amino Aciden
dc.titlePfmrk, a MO15-related protein kinase from Plasmodium falciparum. Gene cloning, sequence, stage-specific expression and chromosome localization.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/8944769en
plymouth.issue3en
plymouth.volume241en
plymouth.publication-statusPublisheden
plymouth.journalEur J Biochemen
dc.identifier.doi10.1111/j.1432-1033.1996.00805.xen
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine
dc.publisher.placeEnglanden
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1111/j.1432-1033.1996.00805.xen
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.typeJournal Article/Reviewen


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