Show simple item record

dc.contributor.authorLi, JLen
dc.contributor.authorBaker, DAen
dc.contributor.authorCox, LSen
dc.date.accessioned2017-11-27T18:46:03Z
dc.date.available2017-11-27T18:46:03Z
dc.date.issued2000-04-25en
dc.identifier.issn0006-3002en
dc.identifier.urihttp://hdl.handle.net/10026.1/10326
dc.description.abstract

A third calcium-dependent protein kinase (CDPK) gene has been isolated from the human malaria parasite Plasmodium falciparum by vectorette technology. The gene consists of five exons and four introns. The open reading frame resulting from removal of the four introns encodes a protein of 562 amino acid residues with a predicted molecular mass of 65.3 kDa. The encoded protein, termed PfCDPK3, consists of four distinct domains characteristic of a member of the CDPK family and displays the highest homology (46% identity and 69% similarity) to PfCDPK2, the second CDPK of P. falciparum. The N-terminal variable domain is rich in serine/threonine and lysine and contains multiple consensus phosphorylation sites for a range of protein kinases. The catalytic domain possesses all conserved motifs of the protein kinase family except for the highly conserved glutamic acid residue in subdomain VIII, which is replaced by a glutamine residue. The sequence of the junction domain comprising 31 amino acid residues is less conserved. The calmodulin-like regulatory domain contains four EF-hand calcium-binding motifs, each consisting of a loop of 12 amino acid residues which is flanked by two alpha-helices. Southern blotting of genomic DNA digests showed that the Pfcdpk3 gene is present as a single copy per haploid genome. A 2900 nucleotide transcript of this gene is expressed specifically in the sexual erythrocytic stage, indicating that PfCDPK3 is involved in sexual stage-specific events. It is proposed that PfCDPK3 may serve as a link between calcium and gametogenesis of P. falciparum.

en
dc.format.extent341 - 349en
dc.languageengen
dc.language.isoengen
dc.subjectAmino Acid Sequenceen
dc.subjectAnimalsen
dc.subjectBase Sequenceen
dc.subjectCalcium-Binding Proteinsen
dc.subjectCloning, Molecularen
dc.subjectGametogenesisen
dc.subjectGenes, Protozoanen
dc.subjectIsoenzymesen
dc.subjectMolecular Sequence Dataen
dc.subjectPlasmodium falciparumen
dc.subjectProtein Kinasesen
dc.subjectProtozoan Proteinsen
dc.subjectRNAen
dc.subjectRestriction Mappingen
dc.subjectSequence Alignmenten
dc.titleSexual stage-specific expression of a third calcium-dependent protein kinase from Plasmodium falciparum.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/10760601en
plymouth.issue1-3en
plymouth.volume1491en
plymouth.publication-statusPublisheden
plymouth.journalBiochim Biophys Actaen
dc.identifier.doi10.1016/s0167-4781(00)00032-4en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine
dc.publisher.placeNetherlandsen
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1016/s0167-4781(00)00032-4en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.typeJournal Article/Reviewen


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record


All items in PEARL are protected by copyright law.
Author manuscripts deposited to comply with open access mandates are made available in accordance with publisher policies. Please cite only the published version using the details provided on the item record or document. In the absence of an open licence (e.g. Creative Commons), permissions for further reuse of content should be sought from the publisher or author.
Theme by 
Atmire NV