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dc.contributor.authorHao, J-Men
dc.contributor.authorChen, J-Zen
dc.contributor.authorSui, H-Men
dc.contributor.authorSi-Ma, X-Qen
dc.contributor.authorLi, G-Qen
dc.contributor.authorLiu, Cen
dc.contributor.authorLi, J-Len
dc.contributor.authorDing, Y-Qen
dc.contributor.authorLi, J-Men
dc.date.accessioned2017-11-27T16:51:17Z
dc.date.available2017-11-27T16:51:17Z
dc.date.issued2010-03en
dc.identifier.urihttp://hdl.handle.net/10026.1/10314
dc.description.abstract

To understand the molecular mechanisms of metastasis and prognosis of colorectal cancer (CRC), we isolated single cell-derived progenies (SCPs) from SW480 cells in vitro and compared their metastatic potential in an orthotopic CRC tumour model in vivo. Two groups of SCPs with the capability of high and low metastasis, respectively, were obtained. By analysing the gene expression profiles of high (SCP51), low (SCP58) metastatic SCPs, and their parental cell line (SW480/EGFP), we demonstrated that 143 genes were differentially expressed either between SCP51 and SCP58 or between SCP58 and SW480/EGFP. Gene-annotation enrichment analysis of DAVID revealed 80 genes in the top ten clusters of the analysis (gene enrichment score > 1). Of the 80-gene set, 32 genes are potentially involved in metastasis, as revealed by Geneclip. Five putative metastatic genes (LYN, SDCBP, MAP4K4, DKK1, and MID1) were selected for further validations. Immunohistochemical analysis in a cohort of 181 CRC clinical samples showed that the individual expression of LYN, MAP4K4, and MID1, as well as the five-gene signature, was closely correlated with lymph node metastasis in CRC patients. More importantly, the individual expression of LYN, MAP4K4, SDCBP, and MID1, as well as the five-gene signature, was significantly correlated with overall survival in CRC patients. Thus, our five-gene signature may be able to predict metastasis and survival of CRC in the clinic, and opens new perspectives on the biology of CRC.

en
dc.format.extent475 - 489en
dc.languageengen
dc.language.isoengen
dc.subjectAnimalsen
dc.subjectCell Proliferationen
dc.subjectColorectal Neoplasmsen
dc.subjectFemaleen
dc.subjectGene Expression Profilingen
dc.subjectHumansen
dc.subjectMaleen
dc.subjectMiceen
dc.subjectMice, Nudeen
dc.subjectNeoplasm Metastasisen
dc.subjectNeoplasm Proteinsen
dc.subjectNeoplasm Transplantationen
dc.subjectNeoplastic Stem Cellsen
dc.subjectOligonucleotide Array Sequence Analysisen
dc.subjectPrognosisen
dc.subjectReverse Transcriptase Polymerase Chain Reactionen
dc.subjectSurvival Analysisen
dc.subjectTumor Cells, Cultureden
dc.titleA five-gene signature as a potential predictor of metastasis and survival in colorectal cancer.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/20077526en
plymouth.issue4en
plymouth.volume220en
plymouth.publication-statusPublisheden
plymouth.journalJ Patholen
dc.identifier.doi10.1002/path.2668en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine
dc.publisher.placeEnglanden
dc.identifier.eissn1096-9896en
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1002/path.2668en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.typeJournal Article/Reviewen


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