Show simple item record

dc.contributor.authorJiao, Ren
dc.contributor.authorBachrati, CZen
dc.contributor.authorPedrazzi, Gen
dc.contributor.authorKuster, Pen
dc.contributor.authorPetkovic, Men
dc.contributor.authorLi, J-Len
dc.contributor.authorEgli, Den
dc.contributor.authorHickson, IDen
dc.contributor.authorStagljar, Ien
dc.date.accessioned2017-11-27T16:35:32Z
dc.date.available2017-11-27T16:35:32Z
dc.date.issued2004-06en
dc.identifier.issn0270-7306en
dc.identifier.urihttp://hdl.handle.net/10026.1/10308
dc.description.abstract

Bloom's syndrome (BS) is a genomic instability disorder characterized by cancer susceptibility. The protein defective in BS, BLM, belongs to the RecQ family of DNA helicases. In this study, we found that BLM interacts with hp150, the largest subunit of chromatin assembly factor 1 (CAF-1), in vitro and in vivo. Colocalization of a proportion of the cellular complement of these two proteins is found at specific nuclear foci coinciding with sites of DNA synthesis in the S phase. This colocalization increases in the presence of agents that damage DNA or inhibit DNA replication. In support of a functional interaction between BLM and CAF-1, we show that BLM inhibits CAF-1-mediated chromatin assembly during DNA repair in vitro. Although CAF-1 activity is not altered in BLM-deficient cells, the absence of BLM does impair the ability of CAF-1 to be mobilized within the nucleus in response to hydroxyurea treatment. Our results provide the first link between BLM and chromatin assembly coupled to DNA repair and suggest that BLM and CAF-1 function in a coordinated way to promote survival in response to DNA damage and/or replication blockade.

en
dc.format.extent4710 - 4719en
dc.languageengen
dc.language.isoengen
dc.subjectAdenosine Triphosphatasesen
dc.subjectChromatin Assembly Factor-1en
dc.subjectChromosomal Proteins, Non-Histoneen
dc.subjectDNA Helicasesen
dc.subjectDNA Repairen
dc.subjectDNA-Binding Proteinsen
dc.subjectHeLa Cellsen
dc.subjectHumansen
dc.subjectIn Vitro Techniquesen
dc.subjectProtein Interaction Mappingen
dc.subjectRecQ Helicasesen
dc.titlePhysical and functional interaction between the Bloom's syndrome gene product and the largest subunit of chromatin assembly factor 1.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/15143166en
plymouth.issue11en
plymouth.volume24en
plymouth.publication-statusPublisheden
plymouth.journalMol Cell Biolen
dc.identifier.doi10.1128/MCB.24.11.4710-4719.2004en
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA01 Clinical Medicine/UoA01 Clinical Medicine
dc.publisher.placeUnited Statesen
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1128/MCB.24.11.4710-4719.2004en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.typeJournal Article/Reviewen


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record


All items in PEARL are protected by copyright law.
Author manuscripts deposited to comply with open access mandates are made available in accordance with publisher policies. Please cite only the published version using the details provided on the item record or document. In the absence of an open licence (e.g. Creative Commons), permissions for further reuse of content should be sought from the publisher or author.
Theme by 
Atmire NV