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dc.contributor.authorPrabhakar, Aen
dc.contributor.authorBishop, AHen
dc.date.accessioned2017-11-17T17:19:22Z
dc.date.available2017-11-17T17:19:22Z
dc.date.issued2014-01en
dc.identifier.urihttp://hdl.handle.net/10026.1/10207
dc.description.abstract

Denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) were used to characterise the changes that occurred in Bacillus cereus group strains present in the phylloplane of clover Trifolium hybridum over 4 months. These strains had previously been analysed by multiple locus sequence typing (MLST). DGGE displayed many equally intense bands which indicated many equally abundant ribotypes. The bacterial community composition was variable and the leaves sampled as little as a week apart were found to have some temporal variability, indicating that diverse phylloplane bacterial communities follow sequential patterns from time to time. The B. cereus group community clearly clustered into early, mid and late branches, possibly due to multiple successional sequences occurring during growing seasons. The functionally and phylogenetically diverse microbial communities appeared to exhibit predictable successional patterns over shorter time scales. DGGE analysis with the molecular marker rpoB gave better resolution than 16S rRNA amplicons. There were no strong similarities between the dendrograms produced by DGGE, MLST and T-RFLP and the clustering produced by the automated T-RFLP method was variable even between the three restriction enzymes used. The DGGE-MLST method emerged as a superior method to T-RFLP-MLST for rapid typing of bacterial communities.

en
dc.format.extent153 - 161en
dc.languageengen
dc.language.isoengen
dc.subjectBacillusen
dc.subjectBacterial Typing Techniquesen
dc.subjectBiodiversityen
dc.subjectDNA-Directed RNA Polymerasesen
dc.subjectDenaturing Gradient Gel Electrophoresisen
dc.subjectGenetic Variationen
dc.subjectMultilocus Sequence Typingen
dc.subjectPlant Leavesen
dc.subjectPolymorphism, Restriction Fragment Lengthen
dc.subjectRNA, Ribosomal, 16Sen
dc.subjectTrifoliumen
dc.titleComparative studies to assess bacterial communities on the clover phylloplane using MLST, DGGE and T-RFLP.en
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/23861044en
plymouth.issue1en
plymouth.volume30en
plymouth.publication-statusPublisheden
plymouth.journalWorld J Microbiol Biotechnolen
dc.identifier.doi10.1007/s11274-013-1434-xen
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
dc.publisher.placeGermanyen
dcterms.dateAccepted2013-07-10en
dc.identifier.eissn1573-0972en
dc.rights.embargoperiodNot knownen
rioxxterms.versionofrecord10.1007/s11274-013-1434-xen
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2014-01en
rioxxterms.typeJournal Article/Reviewen


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