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dc.contributor.authorCleary, DW
dc.contributor.authorBishop, AH
dc.contributor.authorZhang, L
dc.contributor.authorTopp, E
dc.contributor.authorWellington, EMH
dc.contributor.authorGaze, WH
dc.contributor.editorSmalla K
dc.date.accessioned2017-11-17T17:03:39Z
dc.date.available2017-11-17T17:03:39Z
dc.date.issued2016-10
dc.identifier.issn0168-6496
dc.identifier.issn1574-6941
dc.identifier.urihttp://hdl.handle.net/10026.1/10200
dc.description.abstract

Antimicrobial resistance is one of the most significant challenges facing the global medical community and can be attributed to the use and misuse of antibiotics. This includes use as growth promoters or for prophylaxis and treatment of bacterial infection in intensively farmed livestock from where antibiotics can enter the environment as residues in manure. We characterised the impact of the long-term application of a mixture of veterinary antibiotics alone (tylosin, sulfamethazine and chlortetracycline) on class 1 integron prevalence and soil microbiota composition. Class 1 integron prevalence increased significantly (P < 0.005) from 0.006% in control samples to 0.064% in the treated plots. Soil microbiota was analysed using 16S rRNA gene sequencing and revealed significant alterations in composition. Of the 19 significantly different (P < 0.05) OTUs identified, 16 were of the Class Proteobacteria and these decreased in abundance relative to the control plots. Only one OTU, of the Class Cyanobacteria, was shown to increase in abundance significantly; a curiosity given the established sensitivity of this class to antibiotics. We hypothesise that the overrepresentation of Proteobacteria as OTUs that decreased significantly in relative abundance, coupled with the observations of an increase in integron prevalence, may represent a strong selective pressure on these taxa.

dc.format.extentfiw159-fiw159
dc.format.mediumPrint-Electronic
dc.languageen
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.subject16S rRNA gene
dc.subjectantibiotic resistance
dc.subjectclass 1 integrons
dc.subjectmicrobial diversity
dc.subjectsoil
dc.subjectAnti-Bacterial Agents
dc.subjectChlortetracycline
dc.subjectIntegrons
dc.subjectManure
dc.subjectMicrobiota
dc.subjectPrevalence
dc.subjectRNA, Ribosomal, 16S
dc.subjectSoil
dc.subjectSoil Microbiology
dc.subjectSoil Pollutants
dc.subjectTylosin
dc.titleLong-term antibiotic exposure in soil is associated with changes in microbial community structure and prevalence of class 1 integrons
dc.typejournal-article
dc.typeJournal Article
plymouth.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/27495240
plymouth.issue10
plymouth.volume92
plymouth.publisher-urlhttp://dx.doi.org/10.1093/femsec/fiw159
plymouth.publication-statusPublished
plymouth.journalFEMS Microbiology Ecology
dc.identifier.doi10.1093/femsec/fiw159
plymouth.organisational-group/Plymouth
plymouth.organisational-group/Plymouth/Faculty of Science and Engineering
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA
plymouth.organisational-group/Plymouth/REF 2021 Researchers by UoA/UoA06 Agriculture, Veterinary and Food Science
dc.publisher.placeEngland
dcterms.dateAccepted2016-07-14
dc.identifier.eissn1574-6941
dc.rights.embargoperiodNot known
rioxxterms.versionofrecord10.1093/femsec/fiw159
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2016-10
rioxxterms.typeJournal Article/Review


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