Abstract

Enzymatic extraction methods were evaluated with classical extraction approaches for the determination of arsenic in food. The extraction efficiency for total arsenic was determined by analysing CRM materials DORM-3 fish protein, NIES 106 rice flour and GBW10015 spinach. These were compared with total arsenic concentration determined using microwave-assisted acid digestion and ICP-MS. The total arsenic concentrations in the CRM materials were in good agreement with the certified values. Enzymatic hydrolysis using trypsin has been successfully employed to extract arsenic species in DORM-3 and fish samples. Whilst this method of hydrolysing the proteins worked well for the fish samples, an alternative approach was required to facilitate the digestion of cellulose in plant materials. However, enzymatic extraction using cellulase was found to give unsatisfactory results for both the NIES and GBW10015 CRM materials. Dilute nitric acid (1% HNO3) was found to give a more efficient extraction for arsenic species in the same CRM materials and rice samples. The study was extended to evaluate a range of real samples. Total arsenic concentrations in 13 different types of fish tissue were determined following microwave-assisted acid digestion using nitric acid/hydrogen peroxide, followed by measurement using HPLC-ICP-MS for speciation analysis. The results obtained for fish were in the range of 3.53–98.80 µg g−1 As (dry weight). Similarly, the results of 17 rice samples were in the range of 0.054–0.823 µg g−1. This study demonstrates the importance of selecting an appropriate extraction technique for the quantitative measurement of arsenic species in food.

DOI

10.1080/19440049.2015.1132479

Publication Date

2015-12-11

Publication Title

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT

Volume

33

Issue

3

First Page

433

Last Page

441

ISSN

1944-0049

Embargo Period

2017-02-11

Organisational Unit

School of Geography, Earth and Environmental Sciences

Keywords

Arsenic species, CRM and ICP-MS, enzyme, extraction efficiency

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