ORCID
- Puschendorf, Robert: 0000-0002-8105-171X
Abstract
We conducted a cross-sectional study to detect trypanosome infections of horses and donkeys in the Riyadh Province of Saudi Arabia. DNA was extracted from blood samples collected from 368 horses and 142 donkeys, and subjected to universal first ribosomal internal transcribed spacer region (ITS1)-PCR followed by Trypanosoma evansi species-specific RoTat1.2-PCR. The universal ITS1-PCR revealed T. evansi infection in horses ( n = 12; 3.3%) and donkeys ( n = 4; 2.8%). There was no significant effect of sex or age on the prevalence of trypanosomiasis in horses or donkeys. Application of the RoTat1.2-PCR revealed that the RoTat1.2 VSG gene was absent from the positive ITS1-PCR samples of 3 horses and 1 donkey. This discrepancy could be explained by the circulation of T. evansi type B in Saudi Arabia; however, this suspicion requires confirmation.
DOI
10.1177/1040638718798688
Publication Date
2018-11-01
Publication Title
Journal of Veterinary Diagnostic Investigation
Volume
30
Issue
6
ISSN
1040-6387
Embargo Period
2019-02-12
Organisational Unit
School of Biological and Marine Sciences
Keywords
Equine, ITS1, PCR, RoTat1.2, Saudi Arabia, trypanosomiasis
Recommended Citation
Alanazi, A., Puschendorf, R., Salim, B., Alyousif, M., Alanazi, I., & Al-shehri, H. (2018) 'Molecular detection of equine trypanosomiasis in the Riyadh Province of Saudi Arabia.', Journal of Veterinary Diagnostic Investigation, 30(6). Available at: https://doi.org/10.1177/1040638718798688
