A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in Trichoderma reesei

Authors

Amanda Cristina Campos Antonieto
Karoline Maria Vieira Nogueira
Paula RG de
Luísa Czamanski Nora
Murilo Henrique Anzolini Cassiano
Maria Eugenia Guazzaroni
Fausto Almeida
Silva TA da
Laure Nicolas Annick Ries, School of Biomedical Sciences
Assis LJ de, Peninsula Medical School
Gustavo Henrique Goldman
Roberto Nascimento Silva
Rafael Silva-Rocha

ORCID

• Ries, Laure: 0000-0001-9653-3159

Abstract

In this work, we used a systems biology approach to map new regulatory interactions in Trichoderma reesei controlling the expression of genes encoding cellulase and hemicellulase. By integrating transcriptomics related to complex biomass degradation, we were able to identify a novel transcriptional regulator which is able to activate the expression of these genes in response to two different cellulose sources. In vivo experimental validation confirmed the role of this new regulator in several other processes related to carbon source utilization and nutrient transport. Therefore, this work revealed novel forms of regulatory interaction in this model system for plant biomass deconstruction and also represented a new approach that could be easy applied to other organisms.

DOI

https://doi.org/10.1128/msystems.00161-19

Publication Date

2019-08-27

Publication Title

mSystems

Volume

4

Issue

4

Organisational Unit

School of Biomedical Sciences

Recommended Citation

Antonieto, A., Nogueira, K., de, P., Nora, L., Cassiano, M., Guazzaroni, M., Almeida, F., da, S., Ries, L., de, A., Goldman, G., Silva, R., & Silva-Rocha, R. (2019) 'A Novel Cys2His2 Zinc Finger Homolog of AZF1 Modulates Holocellulase Expression in Trichoderma reesei', mSystems, 4(4). Available at: https://doi.org/https://doi.org/10.1128/msystems.00161-19